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The PREGCARD Test is based on a two-site sandwich immunoassayintended for the determination of hCG in urine. The membrane was pre-coated with goat anti-hCG on the Test Band Region and goat anti-mouse on the Control Region. In performing this test, the patient urine specimen is allowed to react with the coloured conjugate (mouse anti-hCG monoclonal antibody colloidal gold conjugate) which was pre-dried on the strip. The mixture migrates in an upward direction on the strip through capillary action. In a positive result, two distinct pink-rose colour bands will be visible, one in the Test Region and one in the Control Region. To serve as a procedural control, a pink-rose colour band is always visible at the Control Region and this colour band also signifies a negative result. The immunological specificity of the test virtually eliminates cross reactivity interference from the structurally related glycoprotein hormones such as hFSH, hLH and hTSH at physiological levels. |
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