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The pathogenic nature of S.aureus has received wide attention recently especially in cases of suppurative infections, food poisoning and other situations. The identification of S. aureus is generally confirmed by the production of coagulase in the microorganism. The tube test detects free coagulase, an extracellular enzyme produced by S. aureus which activates Prothrombin that clots the plasma used in the assay. The slide test detects bound coagulase (clumping factor), a cell associated factor which interacts with fibrinogen and causes the aggregation of S. aureus. The demonstration of protein A in over 95% of the human strains of S. aureus has provided a basis for another slide procedure using latex particles coated with human plasma. The high affinity of protein A to the Fe portion of plasma immunoglobulin G (IgG), and of bound coagulase for fibrinogen in plasma allows rapid agglutination of the coated latex particles in the presence of S. aureus. The PULSE SA TEST incorporates the use of specially coated latex particles that will form aggregates in the presence of both protein A and clumping factor as a basis for identifying S.aureus form the other staphylococci. |
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