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ANA-9-Line

《Specification 》:

Article Number: ORG 710

《Product Introduce 》:

ANA-9-Line Immunoblot assay is a membrane based enzyme immunoassay for the semi-quantitative measurement of IgG class autoantibodies to extractable nuclear antigens SS-A 52, SS-A 60, SS-B, RNP/Sm, Sm, centromere B, Jo-1, Scl-70 and ribosomal P proteins in human serum or plasma. The assay is intended for professional in vitro diagnostic use only.

Features & Functions

 

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The ORGENTEC ANA-9-Line is a semi-quantitative membrane-fixed immunoblot for the detection of autoantibodies against various nuclear antigens. This blot test is used for the diagnosis and differential diagnosis of rheumatic diseases.

The nine purified antigens are deposited as bands onto a nitrocellulose strip. Confirmation of the bound antibodies occurs by staining through an enzyme-catalysed substrate reaction.

The ANA-9-Line blot is thus an important and easy-to-use test system for the detection of autoantibodies that are typical of the various rheumatic autoimmune diseases and that function as partial markers for the individual diseases. The ANA-9-Line is particularly valuable in the differentiation of the autoantibody profile after ELISA screening or immunofluorescence tests.

Automated evaluation of immunoblots with iVISION Scanware

The iVISION Scanware makes it possible to automate the evaluation of immunoblots. The software allows these effective and robust test systems to be objectively assessed and the laboratory test results efficiently stored and archived.

 


Technical Data

    Blo
  • Blot


 
Tests   8 or 16 strips
     
 
Productnumber   ORG 710-08 ORG 710-16
     
Calculation   semiquantitative
     
Cut-off   calibration strip
     
Calibration   arbitrary, CDC sera
     
Substrate   NBT / BCIP
     
Antigens   SS-A 52 (Ro 52), SS-A 60 (Ro 60), SS-B (La), Sm, RNP/Sm, Scl-70, Jo-1, centromere B, Rib-P

 

 

Accessories

• Pipettes for 10µl and 1000µl

• Rocking Platform

• iVISION Scanware

 


Downloads

ANA-9-Line Instructions english

ANA-9-Line MSDS english

ORGENTEC Diagnostika Immunoblot Test System Video english


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