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Nucleo-9-Line

《Specification 》:

Article Number: ORG 711

《Product Introduce 》:

Nucleo-9-Line Immunoblot assay is a membrane based enzyme immunoassay for the semi-quantitative measurement of IgG class autoantibodies to dsDNA, nucleosomes, SS-A (52 a 60 kDa), SS-B, Sm, RNP/Sm, Scl-70, Jo-1 and centromere B in human serum or plasma. The assay is intended for professional in vitro diagnostic use only.

Features & Functions

 

ORGENTEC Diagnostika Logo

The Nucleo-9-Line test by ORGENTEC is a membrane-fixed immunoblot for the detection of autoantibodies against various nuclear antigens (ANA). This test system allows for the semi-quantitative detection of autoantibodies typical of various rheumatic diseases.

The nine purified antigens are individually applied as bands on a nitrocellulose test strip. The detection of bound antibodies is made possible by colour changes induced by an enzyme-catalysed substrate reaction.

The Nucleo-9-Line blot is an important and easy-to-use test system for the further differentiation of ANA after ELISA screening or immunofluorescence tests.

Automated evaluation of immunoblots with iVISION Scanware

The iVISION Scanware makes it possible to automate the evaluation of immunoblots. The software allows these effective and robust test systems to be objectively assessed and the laboratory test results efficiently stored and archived.

 


Technical Data

  • Blot


 
Tests   8 or 16 strips
     
 
Productnumber   ORG 711-08 ORG 711-16
     
Calculation   semiquantitative
     
Cut-off   calibration strip
     
Calibration   arbitrary, CDC sera
     
Substrate   NBT / BCIP
     
Antigens   dsDNA, SS-A (Ro), SS-B (La), nucleosomes, centromere B, Sm, RNP/Sm, Scl-70, Jo-1

 

 

Accessories

• Pipettes for 10µl and 1000µl

• Rocking Platform

iVISION Scanware

 


Downloads

Nucleo-9-Line Instructions english

Nucleo-9-Line MSDS english

ORGENTEC Diagnostika Immunoblot Test System Video english


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